A Project can have multiple SpriteA 2D graphic objects. If you are used to working in 3D, Sprites are essentially just standard textures but there are special techniques for combining and managing sprite textures for efficiency and convenience during development. More info
See in Glossary Atlases for different purposes (for example, Variant Atlases with lower-resolution Textures for hardware with different limitations). If you enable all available Sprite Atlases, you might encounter conflicts (refer to Resolving different Sprite Atlas scenarios for more information).
To prevent these issues, properly prepare Sprite Atlases for distribution with the following steps:
Unity includes Sprite Atlases in a Project’s build by default, and automatically loads them at run time. Clear the Include in Build setting of the selected Sprite Atlas to disable this behavior.
If ‘Include in Build’ is disabled, Unity still packs the Sprite Atlas into a *.spriteatlas file in the Project’s AssetsAny media or data that can be used in your game or Project. An asset may come from a file created outside of Unity, such as a 3D model, an audio file or an image. You can also create some asset types in Unity, such as an Animator Controller, an Audio Mixer or a Render Texture. More info
See in Glossary folder. However, Sprites which reference Textures in an disabled Sprite Atlas appear invisible as the reference Texture is not available or loaded. Unity does not include the disabled Sprite Atlas in the Project’s published build, and does not automatically load it at run time. To do so, a script is required to load the Sprite Atlas via Late Binding.